PB1

(0) No Reviews yet
$40.00
SKU: PB1
View product citations for antibody PB1 on CiteAb

In Stock

Available: 47

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-5
Hybridoma Cells Available: Yes
Antigen Species: hamster
Depositor: Juliano, R.L.
Isotype: MIgG1
Antigen Sequence: 140 kDa α5 integrin
Host Species: mouse
Depositors Institution: University of North Carolina Chapel Hill
Positive Tested Species Reactivity:  
Depositors Notes: Specificity note: no cross reaction. Functional effects: inhibits adhesion of CHO to fibronectin coated substrata but not vitronectin, laminin or sera coated.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: trypsinized CHO (Chinese hamster ovary) cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P2x63.Ag8
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528300 
Additional Information:
Recommended Applications: Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PB1 was deposited to the DSHB by Juliano, R.L. (DSHB Hybridoma Product PB1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • FB References
  • All References
  • Initial Publication
    FB References

    Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration.
    Rentero C
    The Journal of biological chemistry 291.3 (2016 Jan 15): 1320-35.

    All References

    Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration.
    Rentero C
    The Journal of biological chemistry 291.3 (2016 Jan 15): 1320-35.

    Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.
    Juliano RL
    Science (New York, N.Y.) 228.4706 (1985 Jun 21): 1448-51.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review