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View product citations for antibody PCRP-ANKHD1-1B3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Ankyrin repeat and KH domain containing 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as HIV-1 Vpr-binding ankyrin repeat protein.
Antigen Molecular Weight: 269.46 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ANKHD1
Immunogen: Recombinant protein
Alternate Gene Names: FLJ10042, FLJ11979, FLJ14127, FLJ20288, KIAA1085, MASK
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: Q8IWZ3 
Immunogen Sequence: Full length
Entrez Gene ID: 54882 
Additional Characterization:
Antibody Registry ID: AB_2618405 
Additional Information: RRID:AB_2618405
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-ANKHD1-1B3 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-ANKHD1-1B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Nature methods
    Blackshaw S
    Nature methods 15.5 (2018 May): 330-338.

    All References

    Nature methods
    Blackshaw S
    Nature methods 15.5 (2018 May): 330-338.

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