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View product citations for antibody PCRP-BRD3-1C12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Bromodomain containing 3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG1
Depositors Institution: National Institutes of Health
Host Species: mouse
Gene: BRD3
Positive Tested Species Reactivity: Human
Alternate Gene Names: KIAA0043, ORFX, RING3L
Antigen Molecular Weight: 79.54 kDa
Clonality: Monoclonal
Predicted Species Reactivity: Human, Mouse, Rat
Epitope Mapped: Yes
Immunogen: Recombinant peptide
Epitope Location or Sequence: a.a. 254-437
Myeloma Strain: SP2/0-Ag14
Immunogen Sequence: a.a. 254-437
Uniprot ID: Q15059 
Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program. RRID:AB_2618450
Entrez Gene ID: 8019 
Antibody Registry ID: AB_2618450 
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-BRD3-1C12 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-BRD3-1C12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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