PCRP-CHEK2-1A4

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$40.00
SKU: PCRP-CHEK2-1A4
View product citations for antibody PCRP-CHEK2-1A4 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Checkpoint kinase 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2b
Depositors Institution: Protein Capture Reagents Program, produced by JHU/CDI
Host Species: mouse
Depositors Notes: This antibody recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis.
Positive Tested Species Reactivity: Human
Gene: CHEK2
Antigen Molecular Weight: 60.91
Alternate Gene Names: CDS1, CHK2, HuCds1, PP1425, RAD53
Immunogen: Recombinant protein
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: O96017 
Immunogen Sequence: Protein Domain
Entrez Gene ID: 11200 
Additional Information: RRID:AB_2618497
Antibody Registry ID: AB_2618497 
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-CHEK2-1A4 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-CHEK2-1A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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