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View product citations for antibody PCRP-ELF3-8F6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ELF3; E74-like factor 3 (ets domain transcription factor, epithelial-specific )
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG1
Depositors Institution: National Institutes of Health
Host Species: mouse
Gene: ELF3
Positive Tested Species Reactivity: Human
Alternate Gene Names: EPR-1, ERT, ESE-1, ESX
Antigen Molecular Weight: 41.45
Clonality: Monoclonal
Predicted Species Reactivity: Human, Rat
Epitope Mapped: No
Immunogen: Recombinant protein
Immunogen Sequence: a.a.45-132
Myeloma Strain: SP2/0-Ag14
Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program RRID: AB_2618602
Uniprot ID: P78545 
Entrez Gene ID: 1999 
Antibody Registry ID: AB_2618602 
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-ELF3-8F6 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-ELF3-8F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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