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View product citations for antibody PCRP-FOXA2-3A2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Hepatocyte nuclear factor 3-beta
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: The protein recognized by this antibody is also known as Forkhead box A2.
Positive Tested Species Reactivity: Human, Mouse
Gene: FOXA2
Antigen Molecular Weight: 48.31 kDa
Alternate Gene Names: HNF3B
Predicted Species Reactivity: Human, Mouse
Clonality: Monoclonal
Immunogen: Recombinant Peptide
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Immunogen Sequence: a.a. 142-287
Uniprot ID: Q9Y261 
Additional Information: RRID: AB_2618625
Entrez Gene ID: 3170 
Antibody Registry ID: AB_2618625 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-FOXA2-3A2 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-FOXA2-3A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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