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View product citations for antibody PCRP-GATA4-1A7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: GATA4; GATA binding protein 4
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Protein Capture Reagents Program, produced by JHU/CDI
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a transcription activator that is important in cardiac development.
Antigen Molecular Weight: 44.57 kDa
Human Protein Atlas:  
Predicted Species Reactivity: Human, Mouse
Gene: GATA4
Immunogen: Recombinant protein domain
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: P43694 
Immunogen Sequence: a.a. 262-321
Entrez Gene ID: 2626 
Additional Characterization:  
Antibody Registry ID: AB_2618645 
Additional Information: RRID: AB_2618645
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-GATA4-1A7 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-GATA4-1A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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