PCRP-GLI1-1E6

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$40.00
SKU: PCRP-GLI1-1E6
View product citations for antibody PCRP-GLI1-1E6 on CiteAb

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Available: 7

DSHB Data Sheet

Catalog Fields

Antigen: GLI family zinc finger 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2b
Depositors Institution: Protein Capture Reagents Program, produced by JHU/CDI
Host Species: mouse
Depositors Notes: This antibody recognizes a protein that is activated by the sonic hedgehog cascade and inhibited by p53.
Positive Tested Species Reactivity: Human, Mouse
Gene: GLI1
Antigen Molecular Weight: 117.9
Alternate Gene Names: GLI
Immunogen: Recombinant protein
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: P08151 
Immunogen Sequence: Protein Domain
Entrez Gene ID: 2735 
Additional Information: RRID: AB_2618651
Antibody Registry ID: AB_2618651 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-GLI1-1E6 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-GLI1-1E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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