PCRP-ISL1-1A9

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SKU: PCRP-ISL1-1A9

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DSHB Data Sheet

Catalog Fields

Product Name/ID: PCRP-ISL1-1A9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ISL1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618775 
Uniprot ID: P61371 
RRID:  
Entrez Gene ID: 3670 
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: Protein Domain
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: ISL1; ISL LIM homeobox 1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/8/2015
Antigen Molecular Weight: 39.04 kDa
Depositor: Common Fund – Protein Capture Reagents Program
Antigen Sequence:
Depositor Institution: National Institutes of Health
Antigen Species: Human
Depositor Notes: This antibody recognizes the TF that regulates insulin, glucagon and somatostatin gene expression.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: RRID: AB_2618775
Predicted Species Reactivity: Chick, Human, Mouse, Rat, Xenopus, Zebrafish
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-ISL1-1A9 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-ISL1-1A9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IHC References
  • All References
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