PCRP-KLF12-1H9

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$40.00
SKU: PCRP-KLF12-1H9
View product citations for antibody PCRP-KLF12-1H9 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Kruppel-like factor 12
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2b
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: This antigen is a repressor of the AP-2-alpha gene.
Positive Tested Species Reactivity: Human
Gene: KLF12
Antigen Molecular Weight: 44.24 kDa
Alternate Gene Names: AP-2rep, AP2REP, HSPC122
Immunogen: Recombinant protein
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Immunogen Sequence: Full length
Uniprot ID: Q9Y4X4 
Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program. RRID:AB_2722223
Entrez Gene ID: 11278 
Antibody Registry ID: AB_2722223 
Recommended Applications: ELISA, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-KLF12-1H9 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-KLF12-1H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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