PCRP-NFE2L2-1D12

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$40.00
SKU: PCRP-NFE2L2-1D12
View product citations for antibody PCRP-NFE2L2-1D12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: NFE2L2; Nuclear factor, erythroid 2-like 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG1
Depositors Institution: National Institutes of Health
Host Species: mouse
Gene: NFE2L2
Positive Tested Species Reactivity: Human, Mouse
Alternate Gene Names: NRF2
Antigen Molecular Weight: 67.83
Clonality: Monoclonal
Immunogen: Recombinant protein
Immunogen Sequence: a.a. 440-523
Uniprot ID: Q16236 
Additional Information: This antibody has been reported to recognize mouse NFE2L2 protein by western blot. RRID: AB_2618882
Entrez Gene ID: 4780 
Antibody Registry ID: AB_2618882 
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-NFE2L2-1D12 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-NFE2L2-1D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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