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View product citations for antibody PCRP-PRDM1-2B9 on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: PR domain zinc finger protein 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Common Fund – Protein Capture Reagents Program
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This transcription factor is a repressor of beta-interferon gene expression.
Antigen Molecular Weight: 114.8
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PRDM1
Immunogen: Recombinant protein
Alternate Gene Names: BLIMP1, PRDI-BF1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: O75626 
Immunogen Sequence: Full length
Entrez Gene ID: 639 
Additional Characterization:  
Antibody Registry ID: AB_2618985 
Additional Information: RRID: AB_2618985
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-PRDM1-2B9 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-PRDM1-2B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    The DNA double-strand "breakome" of mouse spermatids.
    Boissonneault G
    Cellular and molecular life sciences : CMLS 75.15 (2018 Aug): 2859-2872.

    All References

    The DNA double-strand "breakome" of mouse spermatids.
    Boissonneault G
    Cellular and molecular life sciences : CMLS 75.15 (2018 Aug): 2859-2872.

    A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
    Blackshaw S
    Nature methods 15.5 (2018 May): 330-338.

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