PCRP-PRDM1-2B9

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$40.00
SKU: PCRP-PRDM1-2B9
View product citations for antibody PCRP-PRDM1-2B9 on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: PR domain zinc finger protein 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: This transcription factor is a repressor of beta-interferon gene expression.
Positive Tested Species Reactivity: Human, Mouse
Gene: PRDM1
Antigen Molecular Weight: 114.8
Alternate Gene Names: BLIMP1, PRDI-BF1
Immunogen: Recombinant protein
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: O75626 
Immunogen Sequence: Full length
Entrez Gene ID: 639 
Additional Information: RRID: AB_2618985
Antibody Registry ID: AB_2618985 
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-PRDM1-2B9 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-PRDM1-2B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    The DNA double-strand "breakome" of mouse spermatids.
    Boissonneault G
    Cellular and molecular life sciences : CMLS 75.15 (2018 Aug): 2859-2872.

    All References

    The DNA double-strand "breakome" of mouse spermatids.
    Boissonneault G
    Cellular and molecular life sciences : CMLS 75.15 (2018 Aug): 2859-2872.

    A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
    Blackshaw S
    Nature methods 15.5 (2018 May): 330-338.

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