PCRP-ZIC1-1E3

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$40.00
SKU: PCRP-ZIC1-1E3
View product citations for antibody PCRP-ZIC1-1E3 on CiteAb

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Available: 16

DSHB Data Sheet

Catalog Fields

Antigen: ZIC1; Zic family member 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: This antibody recognizes a transcription factor involved in early neural development.
Positive Tested Species Reactivity: Human
Gene: ZIC1
Antigen Molecular Weight: 48.31 kDa
Alternate Gene Names: ZIC, ZNF201
Predicted Species Reactivity: Chick, Human, Xenopus, Zebrafish
Clonality: Monoclonal
Immunogen: Recombinant peptide
Immunogen Sequence: a.a. 200-385
Myeloma Strain: SP2/0-Ag14
Additional Information: RRID:AB_2619296
Uniprot ID: Q15915 
Entrez Gene ID: 7545 
Antibody Registry ID: AB_2619296 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-ZIC1-1E3 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-ZIC1-1E3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • All References
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