PL1

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$40.00
SKU: PL1
View product citations for antibody PL1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: P-selectin glycoprotein ligand-1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: McEver, R.P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oklahoma Medical Research Foundation
Positive Tested Species Reactivity: Human
Depositors Notes: PL1 blocks binding of P-selectin to neutrophils, monocytes, lymphocytes and HL-60 cells.
Antigen Molecular Weight: 43.2 kDa/ 120kDa glycosylated reduced/250 kD native
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SELPLG
Immunogen: Purified neutrophil Psgl-1p
Alternate Gene Names: CD162, CLA, PSGL-1, PSGL1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-653
Epitope Location or Sequence: N-terminal a.a. 42-57
Uniprot ID: Q14242 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 6404 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PL1 was deposited to the DSHB by McEver, R.P. (DSHB Hybridoma Product PL1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
    McEver RP
    The Journal of cell biology 128.4 (1995 Feb): 661-71.

    IF References

    P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
    McEver RP
    The Journal of cell biology 128.4 (1995 Feb): 661-71.

    WB References

    P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
    McEver RP
    The Journal of cell biology 128.4 (1995 Feb): 661-71.

    FB References

    P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
    McEver RP
    The Journal of cell biology 128.4 (1995 Feb): 661-71.

    FACS References

    P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
    McEver RP
    The Journal of cell biology 128.4 (1995 Feb): 661-71.

    Epitope Map References

    Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies.
    McEver RP
    The Journal of biological chemistry 271.11 (1996 Mar 15): 6342-8.

    Affinity and kinetic analysis of P-selectin binding to P-selectin glycoprotein ligand-1.
    McEver RP
    The Journal of biological chemistry 273.49 (1998 Dec 4): 32506-13.

    All References

    P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
    McEver RP
    The Journal of cell biology 128.4 (1995 Feb): 661-71.

    Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies.
    McEver RP
    The Journal of biological chemistry 271.11 (1996 Mar 15): 6342-8.

    Affinity and kinetic analysis of P-selectin binding to P-selectin glycoprotein ligand-1.
    McEver RP
    The Journal of biological chemistry 273.49 (1998 Dec 4): 32506-13.

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