Product Name/ID: Pav L24
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: pav
Ab Isotype: MIgG2a
Entrez Gene ID: 38515
Immunogen: Drosophila Pavarotti
Immunogen Sequence: amino acids 208–687
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: No
Antigen Name: His-tagged Pavarotti
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/23/2023
Antigen Molecular Weight: 100.6 kD
Depositor: Parkhurst, S.
Depositor Institution: Fred Hutchinson Cancer Center
Antigen Species: Drosophila
Depositor Notes: Pavarotti is required for nuclear envelope bud formation. Pav knockdown experiments using RNAi constructs demonstrate the specificity of Pav L24.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Pavarotti is found in the Centralspindlin complex and associates with WASH to regulate nuclear envelope budding.
Additional Characterization: https://www.biorxiv.org/content/10.1101/2022.11.16.516846v1.full
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Pav L24 was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product Pav L24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.