Phospho-TDP43 (Ser409/410)
Catalog Fields
Clone ID/Product Name: Phospho-TDP43 (Ser409/410)
Available to For-Profits: No
Alternate Antibody Name: 26H10
Gene Symbol: TARDBP
Ab Isotype: Rabbit IgG
Gene Name:
Uniprot ID: Q13148
Entrez Gene ID: 23435
Clonality: Monoclonal
Immunogen: Peptides
Clone:
Immunogen Sequence: Ac-CSMDSK[pS][pS]GWGM-OH
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: TDP-43 phosphorylated at Serine 409/410 sites
Epitope Location or Sequence: Ac-CSMDSK[pS][pS]GWGM-OH
Alternate Antigen Name:
Deposit Date: 8/1/2024
Antigen Molecular Weight: ~45kDa
Depositor: Petrucelli, L, Zhang,Y., Castellanos Otero, P.A‚A
Antigen Sequence:
Depositor Institution: Mayo Foundation for Medical Education and Research
Antigen Species: Human
Depositor Notes: use at approx. 0.1 µg/ml for immunostaining (IHC, IF) and 0.5 µg/ml for immunoblot.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse
Additional Information:
Predicted Species Reactivity:
Human Protein Atlas: https://www.proteinatlas.org/ENSG00000120948-TARDBP
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Phospho-TDP43 (Ser409/410) was deposited to the DSHB by Petrucelli, L, Zhang,Y., Castellanos Otero, P.A‚A (DSHB Hybridoma Product Phospho-TDP43 (Ser409/410))
Storage and Handling Recommendations
Although many cell products are maintained at 4˚C for years without loss of activity, shelf-life at 4˚C is highly variable. For immediate use, short term storage at 4˚C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20˚C or -80˚C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
Generation and characterization of monoclonal antibodies against pathologically phosphorylated TDP-43.
Zhang YJ
PloS one 19.4 (2024): e0298080.