Clone ID / Name: R40.76
Anti - ZO-1; tight junction protein 1
IF of mouse ZO-1 in Eph4 ZO-1-KO +rescue cells labeled with DSHB antibody R40.76 against tight junction protein 1 (red). Image Credit: D.A. Goodenough, Harvard Medical School, PMID: 31438766, Fig. 3a
Catalog Fields
Identity
Clone ID / Name: R40.76
Alternate Antibody Name: —
Antigen Name: ZO-1; tight junction protein 1
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: Tjp1
Entrez Gene ID: 292994
Antigen Sequence: —
Antigen Species: Mouse
Antigen Mol. Weight: 194kD Predicted; 220k kD on SDS-PAGE
Uniprot ID: P39447
Human Protein Atlas: —
Antibody Properties
Host Species: Rat
Clonality: Monoclonal
Isotype: RIgG2a
Myeloma Strain: P3X63 Ag8U.1
Antibody Registry / RRID: AB_2939035
Available to For-Profits: No
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Liver canalicular-enriched membrane fraction
Immunogen Sequence: Full-length
Epitope Mapped: No
Epitope Location / Seq: alpha domain of ZO-1
Clone: —
Additional Info: R40.76 specificity validated in ZO-1 KO (PMID: 35259394).
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Canine, Mouse
Predicted Reactivity: —
Depositor
Depositor Name: D.A. Goodenough
Institution: Harvard Medical School
Deposit Date: January 27, 2023
Depositor Notes: ZO-1 protein has an apparent molecular weight of 225 kD in mouse tissues and 210 kD in canine-derived MDCK cells as determined by SDS/PAGE immunoblot analysis (PMID: 2452168). The ZO-1 epitope in on the cytoplasmic face at cell-cell membrane contact points.
Recommended Applications
FFPE
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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