(0)No Reviews yet
$50.00 to $600.00
SKU: R40.76

In Stock

Available: 14

DSHB Data Sheet

Catalog Fields

Product Name/ID: R40.76
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: Tjp1
Ab Isotype: RIgG2a
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: P39447 
Entrez Gene ID: 292994 
Clonality: Monoclonal
Immunogen: Liver canalicular-enriched membrane fraction
Immunogen Sequence: Full-length
Myeloma Strain: P3X63 Ag8U.1
Epitope Mapped: No
Antigen Name: ZO-1; tight junction protein 1
Epitope Location or Sequence: alpha domain of ZO-1
Alternate Antigen Name:
Deposit Date: 1/27/2023
Antigen Molecular Weight: 194kD Predicted; 220k kD on SDS-PAGE
Depositor: Goodenough, D.A.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Mouse
Depositor Notes: ZO-1 protein has an apparent molecular weight of 225 kD in mouse tissues and 210 kD in canine-derived MDCK cells as determined by SDS/PAGE immunoblot analysis (PMID: 2452168). The ZO-1 epitope in on the cytoplasmic face at cell-cell membrane contact points.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Mouse
Additional Information: R40.76 specificity validated in ZO-1 KO (PMID: 35259394).
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R40.76 was deposited to the DSHB by Goodenough, D.A. (DSHB Hybridoma Product R40.76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FFPE References
    Epitope Map References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review