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View product citations for antibody RORgt on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Antigen: RORgt
Available to For-Profits: Yes
Antigen Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: Armenian Hamster IgG
Depositor: Littman, D.
Host Species: Armenian hamster
Antigen Sequence:
Positive Tested Species Reactivity: Mouse
Depositors Institution: NYU School of Medicine, Skirball Inst. of Biomolecular Medicine
Antigen Molecular Weight: GST 26 kDa RORgt 22 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: gt (receptor-related orphan receptor) gamma thymus-specific
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP20
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2618104 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RORgt was deposited to the DSHB by Littman, D. (DSHB Hybridoma Product RORgt)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Requirement for RORgamma in thymocyte survival and lymphoid organ development.
    Littman DR
    Science (New York, N.Y.) 288.5475 (2000 Jun 30): 2369-73.

    All References

    Requirement for RORgamma in thymocyte survival and lymphoid organ development.
    Littman DR
    Science (New York, N.Y.) 288.5475 (2000 Jun 30): 2369-73.

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