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View product citations for antibody RORgt on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: RORgt
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Littman, D.
Isotype: Armenian Hamster IgG
Antigen Sequence:
Host Species: Armenian hamster
Depositors Institution: NYU School of Medicine, Skirball Inst. of Biomolecular Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes:
Antigen Molecular Weight: GST 26 kDa RORgt 22 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: gt (receptor-related orphan receptor) gamma thymus-specific
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP20
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2618104 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RORgt was deposited to the DSHB by Littman, D. (DSHB Hybridoma Product RORgt)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Requirement for RORgamma in thymocyte survival and lymphoid organ development.
    Littman DR
    Science (New York, N.Y.) 288.5475 (2000 Jun 30): 2369-73.

    All References

    Requirement for RORgamma in thymocyte survival and lymphoid organ development.
    Littman DR
    Science (New York, N.Y.) 288.5475 (2000 Jun 30): 2369-73.

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