Ror2

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SKU: Ror2

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Ror2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Ror2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_10804796 
Uniprot ID: Q9Z138 
RRID:  
Entrez Gene ID: 26564 
Clonality: Monoclonal
Immunogen: Ror2-MBP fusion protein
Clone:
Immunogen Sequence: NT 2535-2835/ C-terminal 100 amino acids
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Receptor tyrosine kinase-like orphan receptor 2/Ror2
Epitope Location or Sequence: C-terminus
Alternate Antigen Name:
Deposit Date: 9/2/2010
Antigen Molecular Weight: 105 kDa
Depositor: Nusse, R.
Antigen Sequence:
Depositor Institution: Stanford University / HHMI
Antigen Species: Mouse
Depositor Notes: This antibody does not cross react with Ror1. The antigen is also known as neurotrophic tyrosine kinase, receptor-related 2 and is involved in cartilage growth, growth plate development and hair follicle patterning.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ror2 was deposited to the DSHB by Nusse, R. (DSHB Hybridoma Product Ror2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
    Nusse R
    The Journal of biological chemistry 284.44 (2009 Oct 30): 30167-76.

    IF References

    Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
    Nusse R
    The Journal of biological chemistry 284.44 (2009 Oct 30): 30167-76.

    Ror2 regulates branching, differentiation, and actin-cytoskeletal dynamics within the mammary epithelium.
    Rosen JM
    The Journal of cell biology 208.3 (2015 Feb 2): 351-66.

    Wnt signaling gradients establish planar cell polarity by inducing Vangl2 phosphorylation through Ror2.
    Yang Y
    Developmental cell 20.2 (2011 Feb 15): 163-76.

    WB References
    IHC References
    IP References
    ELISA References

    Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
    Nusse R
    The Journal of biological chemistry 284.44 (2009 Oct 30): 30167-76.

    FFPE References

    Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
    Nusse R
    The Journal of biological chemistry 284.44 (2009 Oct 30): 30167-76.

    Ror2 regulates branching, differentiation, and actin-cytoskeletal dynamics within the mammary epithelium.
    Rosen JM
    The Journal of cell biology 208.3 (2015 Feb 2): 351-66.

    Wnt5a can both activate and repress Wnt/β-catenin signaling during mouse embryonic development.
    Nusse R
    Developmental biology 369.1 (2012 Sep 1): 101-14.

    Epitope Map References

    Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
    Nusse R
    The Journal of biological chemistry 284.44 (2009 Oct 30): 30167-76.

    All References

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