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SKU: Rpb3-p

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Available: 17

DSHB Data Sheet

Catalog Fields

Product Name/ID: Rpb3
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: Polr2C
Ab Isotype: Rabbit IgG
Gene Symbol:
Antibody Registry ID: AB_2938695 
Uniprot ID: O97183 
Entrez Gene ID: 34796 
Clonality: Polyclonal
Immunogen: His-tag fusion protein
Immunogen Sequence: Full-length
Myeloma Strain: N.A.
Epitope Mapped: No
Antigen Name: RNA polymerase II subunit C
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/18/2023
Antigen Molecular Weight: 31.2 kDa
Depositor: Gilmour, D.S.
Antigen Sequence:
Depositor Institution: Penn State Univ.
Antigen Species: Drosophila
Depositor Notes: This antisera is good for immunofluorescence staining polytene chromosomes. An initial dilution of 1:200 is recommended.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: This antiserum is also validated for permanganate-ChIP-seq.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization: https://flybase.org/reports/FBgn0026373 
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rpb3 was deposited to the DSHB by Gilmour, D.S. (DSHB Hybridoma Product Rpb3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

3 References

  • Initial Publication
  • WB References
  • ChIP References
  • All References
  • Initial Publication
    WB References
    ChIP References
    All References

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