SMED 3D4

(0) No Reviews yet
$40.00
SKU: SMED 3D4
View product citations for antibody SMED 3D4 on CiteAb

In Stock

Available: 42

DSHB Data Sheet

Catalog Fields

Antigen: Marginal/anterior secretory cells intestine (weak)
Hybridoma Cells Available: Yes
Antigen Species: Schmidtea mediterranea/Planaria
Depositor: Newmark, P.A. / Forsthoefel, D.J. / Waters, F.A.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Illinois
Positive Tested Species Reactivity: Planaria, Schmidtea mediterranea
Depositors Notes: See initial citation for fixation optimization.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: phagocytes, gastrodermal (cell line C1W4) fixed
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp 2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2722481 
Additional Information: RRID:AB_2722481
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SMED 3D4 was deposited to the DSHB by Newmark, P.A. / Forsthoefel, D.J. / Waters, F.A. (DSHB Hybridoma Product SMED 3D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review