SPARC mAb 175

(0)No Reviews yet
$50.00 to $600.00
SKU: SPARC mAb 175

In Stock

Available: 37

DSHB Data Sheet

Catalog Fields

Product Name/ID: SPARC mAb 175
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SPARC
Ab Isotype: MIgG2c
Gene Symbol:
Antibody Registry ID: AB_2617207 
Uniprot ID: P09486 
RRID:  
Entrez Gene ID: 6678 
Clonality: Monoclonal
Immunogen: recombinant SPARC injected into SPARC-null mice
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3-X63-Ag8.653
Epitope Mapped: No
Antigen Name: SPARC (secreted protein acidic cysteine-rich) /osteonectin/BM-40
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/13/2013
Antigen Molecular Weight: 43 kDa
Depositor: Brekken, R.A.
Antigen Sequence:
Depositor Institution: UT Southwestern Medical Center
Antigen Species: Human
Depositor Notes: This antibody blocks SPARC mAb 236 and SPARC mAb 303.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: RRID: AB_2617207
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SPARC mAb 175 was deposited to the DSHB by Brekken, R.A. (DSHB Hybridoma Product SPARC mAb 175)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • All References
  • Initial Publication

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    IHC References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    IP References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    ELISA References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    FFPE References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    FB References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    All References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review