SPARC mAb 293

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$40.00
SKU: SPARC mAb 293
View product citations for antibody SPARC mAb 293 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SPARC (secreted protein acidic cysteine-rich) /osteonectin/BM-40
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Brekken, R.A.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UT Southwestern Medical Center,
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Fixation: frozen, formalin and methy Carnoy's
Antigen Molecular Weight: 43 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SPARC
Immunogen: recombinant SPARC injected into SPARC-null mice
Alternate Gene Names: ON
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653
Epitope Location or Sequence: Follistatin domain including SPARC copper binding sequence (KKGHK)
Uniprot ID: P09486 
Immunogen Sequence: Full length protein
Entrez Gene ID: 6678 
Additional Characterization:  
Antibody Registry ID: AB_2617209 
Additional Information: RRID: AB_2617209
Recommended Applications: ELISA, FFPE, Function Blocking, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SPARC mAb 293 was deposited to the DSHB by Brekken, R.A. (DSHB Hybridoma Product SPARC mAb 293)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    IHC References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    IP References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    ELISA References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    The copper binding domain of SPARC mediates cell survival in vitro via interaction with integrin beta1 and activation of integrin-linked kinase.
    Sage EH
    The Journal of biological chemistry 283.33 (2008 Aug 15): 22826-37.

    FFPE References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    FB References

    SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-β1 activity.
    Brekken RA
    The Journal of cell biology 193.7 (2011 Jun 27): 1305-19.

    Epitope Map References
    All References

    Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Sage EH
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 52.6 (2004 Jun): 723-33.

    The copper binding domain of SPARC mediates cell survival in vitro via interaction with integrin beta1 and activation of integrin-linked kinase.
    Sage EH
    The Journal of biological chemistry 283.33 (2008 Aug 15): 22826-37.

    SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-β1 activity.
    Brekken RA
    The Journal of cell biology 193.7 (2011 Jun 27): 1305-19.

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