SUK 2

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$40.00
SKU: SUK 2
View product citations for antibody SUK 2 on CiteAb

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Available: 5

DSHB Data Sheet

Catalog Fields

Antigen: kinesin heavy chain, stalk region
Hybridoma Cells Available: Yes
Antigen Species: Sea Urchin
Depositor: Scholey, J.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Sea Urchin, Xenopus
Depositors Notes: SUK 2 doesn't cross react with squid, bovine or Drosophila kinesins.
Antigen Molecular Weight: Predicted: 118 kDa; Apparent: 130 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x635-68.653
Epitope Location or Sequence: Stalk region of kinesin heavy chain (aa 578-858)
Uniprot ID: P35978 
Immunogen Sequence: Full length protein
Entrez Gene ID: 373178 
Additional Characterization:  
Antibody Registry ID: AB_528325 
Additional Information: In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUK 2 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product SUK 2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    IF References
    WB References

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
    Scholey JM
    The Journal of cell biology 113.4 (1991 May): 817-33.

    Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
    Scholey JM
    Journal of cell science 101 ( Pt 2). (1992 Feb): 291-301.

    Light chains of sea urchin kinesin identified by immunoadsorption.
    Scholey JM
    Cell motility and the cytoskeleton 16.3 (1990): 204-13.

    Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
    Wordeman LG
    Journal of cell science 101 ( Pt 2). (1992 Feb): 303-13.

    Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
    Elinson RP
    The Journal of cell biology 114.5 (1991 Sep): 1017-28.

    Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
    Scholey JM
    Journal of cell science 103 ( Pt 2). (1992 Oct): 309-20.

    The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
    Scholey JM
    The Journal of biological chemistry 269.2 (1994 Jan 14): 1477-85.

    IHC References
    IP References

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Purification and assay of kinesin from sea urchin eggs and early embryos.
    Scholey JM
    Journal of cell science. Supplement 14. (1991): 109-15.

    Light chains of sea urchin kinesin identified by immunoadsorption.
    Scholey JM
    Cell motility and the cytoskeleton 16.3 (1990): 204-13.

    Epitope Map References
    All References

    Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
    Scholey JM
    The Journal of cell biology 113.4 (1991 May): 817-33.

    Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
    Wordeman LG
    Journal of cell science 101 ( Pt 2). (1992 Feb): 303-13.

    Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
    Elinson RP
    The Journal of cell biology 114.5 (1991 Sep): 1017-28.

    Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
    Scholey JM
    Journal of cell science 103 ( Pt 2). (1992 Oct): 309-20.

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
    Scholey JM
    Journal of cell science 101 ( Pt 2). (1992 Feb): 291-301.

    Light chains of sea urchin kinesin identified by immunoadsorption.
    Scholey JM
    Cell motility and the cytoskeleton 16.3 (1990): 204-13.

    The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
    Scholey JM
    The Journal of biological chemistry 269.2 (1994 Jan 14): 1477-85.

    Purification and assay of kinesin from sea urchin eggs and early embryos.
    Scholey JM
    Journal of cell science. Supplement 14. (1991): 109-15.

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