SUK 2
Catalog Fields
Antigen: kinesin heavy chain, stalk region
Hybridoma Cells Available: Yes
Antigen Species: Sea Urchin
Depositor: Scholey, J.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Sea Urchin, Xenopus
Depositors Notes: SUK 2 doesn't cross react with squid, bovine or Drosophila kinesins.
Predicted Species Reactivity:
Gene:
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x635-68.653
Epitope Location or Sequence: Stalk region of kinesin heavy chain (aa 578-858)
Uniprot ID: P35978
Immunogen Sequence: Full length protein
Entrez Gene ID: 373178
Antibody Registry ID: AB_528325
Additional Information: In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUK 2 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product SUK 2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
Scholey JM
The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.