SUK 2
Catalog Fields
Antigen: kinesin heavy chain, stalk region
Hybridoma Cells Available: Yes
Antigen Species: Sea Urchin
Depositor: Scholey, J.M.
Isotype: MIgG1
Depositors Institution: University of California, Davis
Host Species: mouse
Depositors Notes: SUK 2 doesn't cross react with squid, bovine or Drosophila kinesins.
Positive Tested Species Reactivity: Sea Urchin, Xenopus
Clonality: Monoclonal
Antigen Molecular Weight: Predicted: 118 kDa; Apparent: 130 kDa
Epitope Mapped: Yes
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Epitope Location or Sequence: Stalk region of kinesin heavy chain (aa 578-858)
Myeloma Strain: P3x635-68.653
Immunogen Sequence: Full length protein
Uniprot ID: P35978
Additional Information: In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Entrez Gene ID: 373178
Antibody Registry ID: AB_528325
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUK 2 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product SUK 2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
Scholey JM
The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.