SUK 5

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$40.00
SKU: SUK 5
View product citations for antibody SUK 5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: kinesin heavy chain, tail region
Hybridoma Cells Available: Yes
Antigen Species: Sea Urchin
Depositor: Scholey, J.M.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Bovine, Mouse, Sea Urchin, Xenopus
Depositors Notes: SUK 5 doesn't cross react with squid or Drosophila kinesins.
Antigen Molecular Weight: Predicted: 118 kDa; Apparent: 130 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x635-68.653
Epitope Location or Sequence: Tail region of kinesin heavy chain (aa 859-1031)
Uniprot ID: P35978 
Immunogen Sequence: Full length protein
Entrez Gene ID: 373178 
Additional Characterization:  
Antibody Registry ID: AB_528327 
Additional Information: In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUK 5 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product SUK 5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    IF References
    WB References
    IHC References
    IP References

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Epitope Map References
    All References

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