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View product citations for antibody SUMO 6F2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Small ubiquitin-related modifier (SUMO)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Pelisch, F. / Hay, R.T.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Dundee
Positive Tested Species Reactivity: C. elegans
Depositors Notes: The depositor requests citation of the original paper in any publications using this antibody.
Antigen Molecular Weight: 10.2 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: smo-1
Immunogen: Recombinant SMO-1/IRF2 fusion protein
Alternate Gene Names: smt3, sumo
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2
Epitope Location or Sequence:
Uniprot ID: P55853 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 266820 
Additional Characterization:  
Antibody Registry ID: AB_2618393 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUMO 6F2 was deposited to the DSHB by Pelisch, F. / Hay, R.T. (DSHB Hybridoma Product SUMO 6F2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.
    Hay RT
    Nature communications 5. (2014 Dec 5): 5485.

    Tools to Study SUMO Conjugation in Caenorhabditis elegans.
    Hay RT
    Methods in molecular biology (Clifton, N.J.) 1475. (2016): 233-56.

    IP References

    Tools to Study SUMO Conjugation in Caenorhabditis elegans.
    Hay RT
    Methods in molecular biology (Clifton, N.J.) 1475. (2016): 233-56.

    All References

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