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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: SUMO 6F2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: smo-1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2618393 
Uniprot ID: P55853 
Entrez Gene ID: 266820 
Clonality: Monoclonal
Immunogen: Recombinant SMO-1/IRF2 fusion protein
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2
Epitope Mapped: No
Antigen Name: Small ubiquitin-related modifier (SUMO)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/4/2016
Antigen Molecular Weight: 10.2 kDa
Depositor: Pelisch, F. / Hay, R.T.
Antigen Sequence:
Depositor Institution: University of Dundee
Antigen Species: C. elegans
Depositor Notes: The depositor requests citation of the original paper in any publications using this antibody.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUMO 6F2 was deposited to the DSHB by Pelisch, F. / Hay, R.T. (DSHB Hybridoma Product SUMO 6F2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.
    Hay RT
    Nature communications 5. (2014 Dec 5): 5485.

    Tools to Study SUMO Conjugation in Caenorhabditis elegans.
    Hay RT
    Methods in molecular biology (Clifton, N.J.) 1475. (2016): 233-56.

    IP References

    Tools to Study SUMO Conjugation in Caenorhabditis elegans.
    Hay RT
    Methods in molecular biology (Clifton, N.J.) 1475. (2016): 233-56.

    All References

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