ScRPL3
Catalog Fields
Antigen: ribosomal protein L3
Hybridoma Cells Available: Yes
Antigen Species: Saccharomyces cerevisiae
Depositor: Warner, J.R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Albert Einstein College of Medicine
Positive Tested Species Reactivity: Saccharomyces cerevisiae
Depositors Notes: Species specificity: Saccharomyces cerevisiae; no cross-reaction with mammalian L3. specific for L3 in Western of ribosomes and of cytoplasm. IP note: not IP ribosomes and native protein is nearly insoluble, so IP not feasible.
Predicted Species Reactivity:
Gene: TCM1 or RPL3
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS0
Epitope Location or Sequence:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ScRPL3 was deposited to the DSHB by Warner, J.R. (DSHB Hybridoma Product ScRPL3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation.
Fink GR
The Journal of cell biology 130.6 (1995 Sep): 1283-96.