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SKU: Sp-1B10
View product citations for antibody Sp-1B10 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Sp-BetaC ,Sp-BetaCi_1
Hybridoma Cells Available: Yes
Antigen Species: S. purpuratus
Depositor: Burke, R.D.
Isotype: MIgG2a
Depositors Institution: Department of Biochemistry & Microbiology, University of Victoria
Host Species: mouse
Gene: Sp-BetaC ,Sp-BetaCi_1
Positive Tested Species Reactivity: S. purpuratus
Alternate Gene Names: Spu-020342, Spu-027916
Antigen Molecular Weight: 130 kDa
Clonality: Monoclonal
Immunogen: Synthetic peptide
Immunogen Sequence: a.a. 769-781
Myeloma Strain: SP2/0
Uniprot ID: P91808 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sp-1B10 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product Sp-1B10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    All References

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