Sp-40C

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SKU: Sp-40C

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Sp-40C
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Sp-SoxC
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2753217 
Uniprot ID: W4XGT6 
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: E. coli expressed recombinant protein fragment
Clone:
Immunogen Sequence: 128-368
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Sp-SoxC
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/22/2017
Antigen Molecular Weight: 41 kDa
Depositor: Burke, R.D.
Antigen Sequence:
Depositor Institution: University of Victoria, Department of Biochemistry & Microbiology
Antigen Species: S. purpuratus
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: S. purpuratus
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sp-40C was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product Sp-40C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms.
    Burke RD
    Development (Cambridge, England) 143.2 (2016 Jan 15): 286-97.

    IF References

    Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms.
    Burke RD
    Development (Cambridge, England) 143.2 (2016 Jan 15): 286-97.

    All References

    Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms.
    Burke RD
    Development (Cambridge, England) 143.2 (2016 Jan 15): 286-97.

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