Sp14
Catalog Fields
Clone ID/Product Name: Sp14
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528353
Clonality: Monoclonal
Immunogen: Blastocoelar ECM preparations from 72 h. embryos of Strongylocentrotus purpuratus (sea urchin)
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: microfibrils (sea urchin)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/14/1993
Antigen Molecular Weight: 350 kDa
Depositor: Burke, R.D.
Antigen Sequence:
Depositor Institution: University of Victoria
Antigen Species: sea urchin
Depositor Notes: Binds to microfibrillar components of blastocoelar ECM. Does not immunoprecipitate well & Western blots are weak. extraction of antigen is problematic. Interferes with migration of blastocoelar cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Sea Urchin
Additional Information:
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sp14 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product Sp14)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
0 Reference