Clone ID / Name: T9
Anti - Na-K-Cl cotransporter
Catalog Fields
Identity
Clone ID / Name: T9
Alternate Antibody Name: —
Antigen Name: Na-K-Cl cotransporter
Alternate Antigen Name: NKCC, Solute carrier family 12 members 1 and 2
Gene Symbol: —
Gene Name: SLC12A1, SLC12A2
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: Predicted: 121.4kDa Apparent: 145-205kDa
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: P3x63-Ag8.653
Antibody Registry / RRID: AB_2618107
Available to For-Profits: No
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein
Immunogen Sequence: human NKCC1 C-terminus (Met902-Ser1212)
Epitope Mapped: Yes
Epitope Location / Seq: a.a. 902-1212
Clone: —
Additional Info: RRID:AB_2618107
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Mammal
Predicted Reactivity: —
Depositor
Depositor Name: C. Lytle / B. Forbush III
Institution: Yale University
Deposit Date: November 11, 2014
Depositor Notes: Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T9 is better than T4 in recognizing NKCC2, particularly by IF (PMID: 8572179, 12732642). T9 recpgmozes the 196kDa cotransporter exclusively on western blots [PMID: 8910547].
Recommended Applications
IF
WB
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