TC22-4

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$40.00
SKU: TC22-4
View product citations for antibody TC22-4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Tropomyosin alpha-3 chain isoform 5 splice variant TC22
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Lin, J. J-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Human
Depositors Notes: hTM5 isoform is expressed in normal colon epithelial. TC22 is an alternatively spliced variant of hTM5 with an altered C-terminal sequence (a.a. 222-247) that is strongly associated with colonic neoplasms and carcinoma.
Antigen Molecular Weight: 28.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM3
Immunogen: Synthetic peptide a.a. 223-247
Alternate Gene Names: TM-5
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminal splice variant of TC22
Uniprot ID: P06753-4 
Immunogen Sequence: RLYSQLERNRLLSNELKLTLHDLCD
Entrez Gene ID: 7170 
Additional Characterization:  
Antibody Registry ID:  
Additional Information: US Patents 7285630B2 (2010), RE43,472 E (2012) and 9238040B2 (2016).
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TC22-4 was deposited to the DSHB by Lin, J. J-C. (DSHB Hybridoma Product TC22-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Transformation of benign Barrett's epithelium by repeated acid and bile exposure over 65 weeks: a novel in vitro model.
    Hirshfield K
    International journal of cancer 128.2 (2011 Jan 15): 274-82.

    Effect of eradication of Helicobacter pylori on the histology and cellular phenotype of gastric intestinal metaplasia.
    Das KM
    Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 6.4 (2008 Apr): 409-17.

    ELISA References
    FACS References
    Epitope Map References
    All References

    Transformation of benign Barrett's epithelium by repeated acid and bile exposure over 65 weeks: a novel in vitro model.
    Hirshfield K
    International journal of cancer 128.2 (2011 Jan 15): 274-82.

    Effect of eradication of Helicobacter pylori on the histology and cellular phenotype of gastric intestinal metaplasia.
    Das KM
    Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 6.4 (2008 Apr): 409-17.

    Mesalamine suppresses the expression of TC22, a novel tropomyosin isoform associated with colonic neoplasia.
    Das KM
    Molecular pharmacology 76.1 (2009 Jul): 183-91.

    Isolation and sequencing of a novel tropomyosin isoform preferentially associated with colon cancer.
    Lin JJ
    Gastroenterology 123.1 (2002 Jul): 152-62.

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