TC22-4

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$45.00 to $425.00
SKU: TC22-4
View product citations for antibody TC22-4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Tropomyosin alpha-3 chain isoform 5 splice variant TC22
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Lin, J. J-C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: University of Iowa
Antigen Molecular Weight: 28.7 kDa
Depositors Notes: hTM5 isoform is expressed in normal colon epithelial. TC22 is an alternatively spliced variant of hTM5 with an altered C-terminal sequence (a.a. 222-247) that is strongly associated with colonic neoplasms and carcinoma.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Synthetic peptide a.a. 223-247
Gene: TPM3
Alternate Antibody Name:
Alternate Gene Names: TM-5
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: P06753-4 
Epitope Location or Sequence: C-terminal splice variant of TC22
Entrez Gene ID: 7170 
Immunogen Sequence: RLYSQLERNRLLSNELKLTLHDLCD
Antibody Registry ID: AB_2801366 
Additional Characterization:  
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: US Patents 7285630B2 (2010), RE43,472 E (2012) and 9238040B2 (2016).
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TC22-4 was deposited to the DSHB by Lin, J. J-C. (DSHB Hybridoma Product TC22-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Transformation of benign Barrett's epithelium by repeated acid and bile exposure over 65 weeks: a novel in vitro model.
    Hirshfield K
    International journal of cancer 128.2 (2011 Jan 15): 274-82.

    Effect of eradication of Helicobacter pylori on the histology and cellular phenotype of gastric intestinal metaplasia.
    Das KM
    Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 6.4 (2008 Apr): 409-17.

    ELISA References
    FACS References
    Epitope Map References
    All References

    Transformation of benign Barrett's epithelium by repeated acid and bile exposure over 65 weeks: a novel in vitro model.
    Hirshfield K
    International journal of cancer 128.2 (2011 Jan 15): 274-82.

    Effect of eradication of Helicobacter pylori on the histology and cellular phenotype of gastric intestinal metaplasia.
    Das KM
    Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 6.4 (2008 Apr): 409-17.

    Mesalamine suppresses the expression of TC22, a novel tropomyosin isoform associated with colonic neoplasia.
    Das KM
    Molecular pharmacology 76.1 (2009 Jul): 183-91.

    Isolation and sequencing of a novel tropomyosin isoform preferentially associated with colon cancer.
    Lin JJ
    Gastroenterology 123.1 (2002 Jul): 152-62.

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