TC31-9C12.C9
Catalog Fields
Product Name/ID: TC31-9C12.C9
Available to For-Profits: Yes
Alternate Antibody Name: 9C12
Gene Name: L3
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_1553405
Uniprot ID: P04133
Entrez Gene ID: 2652998
Clonality: Monoclonal
Immunogen: Purified human adenovirus serotype 5
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: P3X63Ag
Epitope Mapped: Yes
Antigen Name: Hexon protein, human adenovirus 5
Epitope Location or Sequence: The epitope is most likely formed by residues from the gap of aa 431–436, which includes HVR8 from the FG1 loop, and possibly by residues from the long gap of aa 136–164, which includes most of HVR1. The G434D mutation disrupts binding of the antibody.
Alternate Antigen Name:
Deposit Date: 9/23/2008
Antigen Molecular Weight: Predicted: 108 kDa; Apparent: 108 kDa (monomer, heat-denatured); > 200kDa (trimer, not heat-denatured)
Depositor: Fayadat-Dilman, L. / Olijve, W.
Antigen Sequence:
Depositor Institution: Schering Plough Biopharma
Antigen Species: human adenovirus C serotype 5
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: human adenovirus 5
Additional Information: Hexon is the major capsid protein. The epitope recognized by TC31-9C12.C9 is likely conformation-specific since this mAb can not bind heat denatured hexon. TC31-9C12.C9 immunoprecipitates the complete set of proteins normally associated with intact Ad5 particles; in contrast, TC31-27F11.C2 (a mAb available in the DSHB collection, isolated from the same fusion that generated TC31-9C12.C9) pulls down only free hexon protein. While TC31-9C12.C9 neutralizes Ad5, TC31-27F11.C2 doesn't. TC31-27F11.C2 is the perfect isotype-matched nonneutralizing control Ab for TC31-9C12.C9.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TC31-9C12.C9 was deposited to the DSHB by Fayadat-Dilman, L. / Olijve, W. (DSHB Hybridoma Product TC31-9C12.C9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Postentry neutralization of adenovirus type 5 by an antihexon antibody.
Ralston R
Journal of virology 78.22 (2004 Nov): 12320-32.