TC31-9C12.C9

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$40.00
SKU: TC31-9C12.C9
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DSHB Data Sheet

Catalog Fields

Antigen: Hexon protein, human adenovirus 5
Hybridoma Cells Available: Yes
Antigen Species: human adenovirus C serotype 5
Depositor: Fayadat-Dilman, L. / Olijve, W.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Schering Plough Biopharma
Positive Tested Species Reactivity: human adenovirus 5
Depositors Notes:
Antigen Molecular Weight: Predicted: 108 kDa; Apparent: 108 kDa (monomer, heat-denatured); > 200kDa (trimer, not heat-denatured)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: L3
Immunogen: Purified human adenovirus serotype 5
Alternate Gene Names:
Alternate Antibody Name: 9C12
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag
Epitope Location or Sequence: The epitope is most likely formed by residues from the gap of aa 431–436, which includes HVR8 from the FG1 loop, and possibly by residues from the long gap of aa 136–164, which includes most of HVR1. The G434D mutation disrupts binding of the antibody.
Uniprot ID: P04133 
Immunogen Sequence: full length protein
Entrez Gene ID: 2652998 
Additional Characterization:  
Antibody Registry ID: AB_1553405 
Additional Information: Hexon is the major capsid protein. The epitope recognized by TC31-9C12.C9 is likely conformation-specific since this mAb can not bind heat denatured hexon. TC31-9C12.C9 immunoprecipitates the complete set of proteins normally associated with intact Ad5 particles; in contrast, TC31-27F11.C2 (a mAb available in the DSHB collection, isolated from the same fusion that generated TC31-9C12.C9) pulls down only free hexon protein. While TC31-9C12.C9 neutralizes Ad5, TC31-27F11.C2 doesn't. TC31-27F11.C2 is the perfect isotype-matched nonneutralizing control Ab for TC31-9C12.C9.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TC31-9C12.C9 was deposited to the DSHB by Fayadat-Dilman, L. / Olijve, W. (DSHB Hybridoma Product TC31-9C12.C9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    IF References
    WB References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    IP References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    Directed evolution of mutator adenoviruses resistant to antibody neutralization.
    Smith JG
    Journal of virology 87.10 (2013 May): 6047-50.

    ELISA References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    A Single Maturation Cleavage Site in Adenovirus Impacts Cell Entry and Capsid Assembly.
    Nemerow GR
    Journal of virology 90.1 (2016 Jan 1): 521-32.

    FB References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    Neutralizing antibody blocks adenovirus infection by arresting microtubule-dependent cytoplasmic transport.
    Nemerow GR
    Journal of virology 82.13 (2008 Jul): 6492-500.

    Directed evolution of mutator adenoviruses resistant to antibody neutralization.
    Smith JG
    Journal of virology 87.10 (2013 May): 6047-50.

    AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization.
    James LC
    Proceedings of the National Academy of Sciences of the United States of America 109.48 (2012 Nov 27): 19733-8.

    Regulation of virus neutralization and the persistent fraction by TRIM21.
    James LC
    Journal of virology 86.16 (2012 Aug): 8482-91.

    TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus.
    James LC
    PLoS pathogens 11.10 (2015 Oct): e1005253.

    Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21.
    James LC
    Proceedings of the National Academy of Sciences of the United States of America 112.32 (2015 Aug 11): 10014-9.

    FACS References

    Mechanism of adenovirus neutralization by Human alpha-defensins.
    Nemerow GR
    Cell host & microbe 3.1 (2008 Jan 17): 11-9.

    Epitope Map References

    CryoEM structure at 9A resolution of an adenovirus vector targeted to hematopoietic cells.
    Stewart PL
    Journal of molecular biology 349.3 (2005 Jun 10): 526-37.

    Directed evolution of mutator adenoviruses resistant to antibody neutralization.
    Smith JG
    Journal of virology 87.10 (2013 May): 6047-50.

    All References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    Neutralizing antibody blocks adenovirus infection by arresting microtubule-dependent cytoplasmic transport.
    Nemerow GR
    Journal of virology 82.13 (2008 Jul): 6492-500.

    Functional genetic and biophysical analyses of membrane disruption by human adenovirus.
    Nemerow GR
    Journal of virology 85.6 (2011 Mar): 2631-41.

    Direct evidence from single-cell analysis that human {alpha}-defensins block adenovirus uncoating to neutralize infection.
    Smith JG
    Journal of virology 84.8 (2010 Apr): 4041-9.

    Tracking viral genomes in host cells at single-molecule resolution.
    Greber UF
    Cell host & microbe 14.4 (2013 Oct 16): 468-80.

    Co-option of Membrane Wounding Enables Virus Penetration into Cells.
    Greber UF
    Cell host & microbe 18.1 (2015 Jul 8): 75-85.

    TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus.
    James LC
    PLoS pathogens 11.10 (2015 Oct): e1005253.

    A direct and versatile assay measuring membrane penetration of adenovirus in single cells.
    Greber UF
    Journal of virology 87.22 (2013 Nov): 12367-79.

    Directed evolution of mutator adenoviruses resistant to antibody neutralization.
    Smith JG
    Journal of virology 87.10 (2013 May): 6047-50.

    A Single Maturation Cleavage Site in Adenovirus Impacts Cell Entry and Capsid Assembly.
    Nemerow GR
    Journal of virology 90.1 (2016 Jan 1): 521-32.

    AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization.
    James LC
    Proceedings of the National Academy of Sciences of the United States of America 109.48 (2012 Nov 27): 19733-8.

    Regulation of virus neutralization and the persistent fraction by TRIM21.
    James LC
    Journal of virology 86.16 (2012 Aug): 8482-91.

    Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21.
    James LC
    Proceedings of the National Academy of Sciences of the United States of America 112.32 (2015 Aug 11): 10014-9.

    Mechanism of adenovirus neutralization by Human alpha-defensins.
    Nemerow GR
    Cell host & microbe 3.1 (2008 Jan 17): 11-9.

    CryoEM structure at 9A resolution of an adenovirus vector targeted to hematopoietic cells.
    Stewart PL
    Journal of molecular biology 349.3 (2005 Jun 10): 526-37.

    Antibody-antigen kinetics constrain intracellular humoral immunity.
    James LC
    Scientific reports 6. (2016 Nov 24): 37457.

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