TCR-3

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SKU: TCR-3-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: TCR-3
Available to For-Profits: No
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2796458 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: No
Antigen Name: TCR alpha/beta
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 88 kDa heterodimer; 48 and 40 kDa reduced
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Chick
Depositor Notes: Hybridoma developed by M.D. Cooper.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chick, Guineafowl, Peacock
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TCR-3 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product TCR-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

3 References

  • Initial Publication
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    A third sublineage of avian T cells can be identified with a T cell receptor-3-specific antibody.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 145.11 (1990 Dec 1): 3547-55.

    IHC References

    A third sublineage of avian T cells can be identified with a T cell receptor-3-specific antibody.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 145.11 (1990 Dec 1): 3547-55.

    Lymphocytic infiltration in the chicken trachea in response to Mycoplasma gallisepticum infection.
    Browning GF
    Microbiology (Reading, England) 146 ( Pt 5). (2000 May): 1223-1229.

    IP References

    A third sublineage of avian T cells can be identified with a T cell receptor-3-specific antibody.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 145.11 (1990 Dec 1): 3547-55.

    FACS References

    A third sublineage of avian T cells can be identified with a T cell receptor-3-specific antibody.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 145.11 (1990 Dec 1): 3547-55.

    The structure of avian CD5 implies a conserved function.
    Vainio O
    Journal of immunology (Baltimore, Md. : 1950) 160.10 (1998 May 15): 4943-50.

    All References

    A third sublineage of avian T cells can be identified with a T cell receptor-3-specific antibody.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 145.11 (1990 Dec 1): 3547-55.

    Lymphocytic infiltration in the chicken trachea in response to Mycoplasma gallisepticum infection.
    Browning GF
    Microbiology (Reading, England) 146 ( Pt 5). (2000 May): 1223-1229.

    The structure of avian CD5 implies a conserved function.
    Vainio O
    Journal of immunology (Baltimore, Md. : 1950) 160.10 (1998 May 15): 4943-50.

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