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View product citations for antibody TMPRSS2 P5H9-A3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Transmembrane serine protease 2
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1, kappa light chain
Depositor: Nelson, P.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: 54 kDa glycosylated full length protein/ 26 kDa cleaved serine protease domain
Depositors Notes: The immunogen sequence (a.a. 350-365) lies within the TMPRSS2 protease domain.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: KLH conjugated -synthetic peptide
Alternate Antibody Name:
Alternate Gene Names: PRSS10
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Uniprot ID: O15393 
Epitope Location or Sequence: a.a. 350-365
Entrez Gene ID: 7113 
Immunogen Sequence: a.a. 350-365
Antibody Registry ID: AB_2205599 
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: Fixation either with 30:70 acetone/methanol mixture or 4% paraformaldehyde in PBS. Successful HIER citrate buffer pH 6.0
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TMPRSS2 P5H9-A3 was deposited to the DSHB by Nelson, P.S. (DSHB Hybridoma Product TMPRSS2 P5H9-A3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    FFPE References
    Epitope Map References
    All References

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