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SKU: TRA-2-54/2J-s
View product citations for antibody TRA-2-54/2J on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: alkaline phosphatase, tissue non-specific isoenzyme
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Andrews, P.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sheffield
Positive Tested Species Reactivity: Baboon, Human, Mandrill, Non-human primates, Rhesus monkey
Depositors Notes: This antibody recognizes alkaline phosphatase isozyme from liver, bone and kidney but does not recognize intestinal or placental alkaline phosphatases.
Antigen Molecular Weight: ~80 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ALPL
Immunogen: Embryonal carcinoma (EC) 2102Ep cells
Alternate Gene Names: HOPS, TNSALP
Alternate Antibody Name: chromosome 1, 80 kDa polypeptide
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8-653
Epitope Location or Sequence:
Uniprot ID: P05186 
Immunogen Sequence: Expanded polyglutamine domain (polyQ)
Entrez Gene ID: 249 
Additional Characterization:
Antibody Registry ID: AB_528074 
Additional Information: This antibody does not react with mouse ALP. RRID:AB_528074
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TRA-2-54/2J was deposited to the DSHB by Andrews, P.W. (DSHB Hybridoma Product TRA-2-54/2J)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    ELISA References
    FACS References
    All References

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