TS1/18.1.2.11.4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: TS1/18.1.2.11.4
Available to For-Profits: Yes
Alternate Antibody Name: TS1/18
Gene Name: ITGB2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2128634 
Uniprot ID: P05107 
RRID:  
Entrez Gene ID: 3689 
Clonality: Monoclonal
Immunogen: Human HLA-DRCTL line cytolytic T-lymphocytes.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Antigen Name: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/4/1989
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Center for Blood Research
Antigen Species: Human
Depositor Notes: Does not cross react with mouse. Binds to all leukocytes. Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: CD18 is a general marker of leukocytes.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1/18.1.2.11.4 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product TS1/18.1.2.11.4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

15 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    IF References

    Role of the LFA-1 molecule in cellular interactions required for antibody production in humans.
    Griscelli C
    Journal of immunology (Baltimore, Md. : 1950) 136.9 (1986 May 1): 3198-203.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    IP References
    FB References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells.
    Ehlers MR
    Infection and immunity 64.12 (1996 Dec): 5373-83.

    Maturation of DC is associated with changes in motile characteristics and adherence.
    Thrasher AJ
    Cell motility and the cytoskeleton 57.2 (2004 Feb): 118-32.

    T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
    Springer TA
    Nature 341.6243 (1989 Oct 19): 619-24.

    Role of the LFA-1 molecule in cellular interactions required for antibody production in humans.
    Griscelli C
    Journal of immunology (Baltimore, Md. : 1950) 136.9 (1986 May 1): 3198-203.

    Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
    Anderson DC
    Journal of immunology (Baltimore, Md. : 1950) 143.10 (1989 Nov 15): 3207-10.

    TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen.
    Levy R
    Journal of immunology (Baltimore, Md. : 1950) 145.7 (1990 Oct 1): 2207-13.

    CD43 interferes with T-lymphocyte adhesion.
    Staunton DE
    Proceedings of the National Academy of Sciences of the United States of America 89.11 (1992 Jun 1): 5001-5.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    FACS References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 166.9 (2001 May 1): 5629-37.

    Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
    Anderson DC
    Journal of immunology (Baltimore, Md. : 1950) 143.10 (1989 Nov 15): 3207-10.

    Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 155.2 (1995 Jul 15): 854-66.

    A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
    Springer TA
    The Journal of experimental medicine 158.6 (1983 Dec 1): 1785-1803.

    A human CD4- T cell leukemia subclone with contact-dependent helper function.
    Lederman S
    Journal of immunology (Baltimore, Md. : 1950) 147.10 (1991 Nov 15): 3389-95.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    All References

    Role of the LFA-1 molecule in cellular interactions required for antibody production in humans.
    Griscelli C
    Journal of immunology (Baltimore, Md. : 1950) 136.9 (1986 May 1): 3198-203.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells.
    Ehlers MR
    Infection and immunity 64.12 (1996 Dec): 5373-83.

    Distinct ligand binding sites in the I domain of integrin alpha M beta 2 that differentially affect a divalent cation-dependent conformation.
    Bajt ML
    The Journal of biological chemistry 270.43 (1995 Oct 27): 25866-71.

    Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 155.2 (1995 Jul 15): 854-66.

    A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
    Springer TA
    The Journal of experimental medicine 158.6 (1983 Dec 1): 1785-1803.

    Maturation of DC is associated with changes in motile characteristics and adherence.
    Thrasher AJ
    Cell motility and the cytoskeleton 57.2 (2004 Feb): 118-32.

    T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
    Springer TA
    Nature 341.6243 (1989 Oct 19): 619-24.

    Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
    Anderson DC
    Journal of immunology (Baltimore, Md. : 1950) 143.10 (1989 Nov 15): 3207-10.

    TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen.
    Levy R
    Journal of immunology (Baltimore, Md. : 1950) 145.7 (1990 Oct 1): 2207-13.

    CD43 interferes with T-lymphocyte adhesion.
    Staunton DE
    Proceedings of the National Academy of Sciences of the United States of America 89.11 (1992 Jun 1): 5001-5.

    Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 166.9 (2001 May 1): 5629-37.

    A human CD4- T cell leukemia subclone with contact-dependent helper function.
    Lederman S
    Journal of immunology (Baltimore, Md. : 1950) 147.10 (1991 Nov 15): 3389-95.

    Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells.
    Van Furth R
    Immunology 74.4 (1991 Dec): 661-9.

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