TS1/18.1.2.11.4
Catalog Fields
Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Springer, T.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Center for Blood Research
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Depositors Notes: Does not cross react with mouse. Binds to all leukocytes. Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting.
Immunogen: Human HLA-DRCTL line cytolytic T-lymphocytes.
Gene: ITGB2
Alternate Antibody Name: TS1/18
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Uniprot ID: P05107
Epitope Location or Sequence:
Entrez Gene ID: 3689
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2128634
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Additional Information: CD18 is a general marker of leukocytes.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1/18.1.2.11.4 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product TS1/18.1.2.11.4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
Springer TA
Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.