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SKU: Tinman-p
View product citations for antibody Tinman on CiteAb

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Available: 91

DSHB Data Sheet

Catalog Fields

Antigen: Muscle-specific homeobox protein tinman
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Frasch, M.
Isotype: Rabbit IgG
Antigen Sequence:
Host Species: rabbit
Depositors Institution: Universitaet Erlangen- Nuernberg
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Recommended dilution 1:1000
Antigen Molecular Weight: 46.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: tin
Immunogen: 6xHis tag fusion protein expressed in E. coli
Alternate Gene Names: msh2, CG7895
Alternate Antibody Name:
Clonality: Polyclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NA
Epitope Location or Sequence:
Uniprot ID: P22711 
Immunogen Sequence: a.a. 1-416
Entrez Gene ID: 42536 
Additional Characterization:  
Antibody Registry ID: AB_2892601 
Additional Information: FBgn0004110
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Tinman was deposited to the DSHB by Frasch, M. (DSHB Hybridoma Product Tinman)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • ChIP References
  • All References
  • Initial Publication
    IF References
    IHC References
    ChIP References
    All References

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