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SKU: Tinman-p
View product citations for antibody Tinman on CiteAb

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Available: 46

DSHB Data Sheet

Catalog Fields

Product Name/ID: Tinman
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: tin
Ab Isotype: Rabbit IgG
Gene Symbol:
Antibody Registry ID: AB_2892601 
Uniprot ID: P22711 
Entrez Gene ID: 42536 
Clonality: Polyclonal
Immunogen: 6xHis tag fusion protein expressed in E. coli
Immunogen Sequence: a.a. 1-416
Myeloma Strain: NA
Epitope Mapped: No
Antigen Name: Muscle-specific homeobox protein tinman
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/30/2021
Antigen Molecular Weight: 46.0 kDa
Depositor: Frasch, M.
Antigen Sequence:
Depositor Institution: Universitaet Erlangen- Nuernberg
Antigen Species: Drosophila
Depositor Notes: Recommended dilution 1:1000
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: FBgn0004110
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Tinman was deposited to the DSHB by Frasch, M. (DSHB Hybridoma Product Tinman)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • IHC References
  • ChIP References
  • All References
  • Initial Publication
    IF References
    IHC References
    ChIP References
    All References

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