Antigen: glycoprotein, Ulocladium
Hybridoma Cells Available: No
Antigen Species: Ulocladium atrum
Depositor: Dewey, F.M.
Host Species: mouse
Depositors Institution: University of Oxford
Positive Tested Species Reactivity: Alternaria, Epicoccum, Stemphylium, Ulocladium
Depositors Notes: group-specific, recognizes all isolates of Ulocladium, Alternaria, Stemphylium and Epicoccum tested but not other fungi or extracts from plants. glycoprotein, the binding site (epitope) is heat and protease stable but sensitive to periodate oxidation. Functional effects: antigen production stimulated by the addition of 1% (w/v) fragments of cyclamen leaves to liquid oat meal broth cultures. IHC notes: the antibody has been used in tests by ELISA to specifically determine quantitatively the levels of U. atrum in cyclamen and strawberry leaves co-infected with Botrytis cinerea. Cell Binding: binds to water soluble product produced in vitro and in planta by species of Ulocladium, Alternaria, Stemphylium and Epicoccum. Immunohistology: The antibody has been used to visualize the fungal hyphae in vitro and in planta by immunofluorescence and TEM. Melanized conidia are not immunolabelled by the antibody.
Predicted Species Reactivity:
Immunogen: phosphate buffered saline extracts from Cyclamen leaves, that had been artificially infected with Ulocladium atrum 385 and then dried at ambient temperature for 10 days
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Y3
Epitope Location or Sequence:
Antibody Registry ID: AB_10658861
Recommended Applications: ELISA, Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
UA-PC3 was deposited to the DSHB by Dewey, F.M. (DSHB Hybridoma Product UA-PC3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).