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DSHB Data Sheet

Catalog Fields

Antigen: LAMP-2 (hamster)
Hybridoma Cells Available: Yes
Antigen Species: Hamster
Depositor: Granger, B.L. / Uthayakumar, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Montana State University
Positive Tested Species Reactivity: Hamster
Depositors Notes: UH1 recognizes native, methanol-fixed, and PLP-fixed hamster antigen. Doesn't recognize rat, mouse or monkey antigens. UH3 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Antigen Molecular Weight: Predicted: 42 kDa; Apparent: 110 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: LAMP2
Immunogen: Membrane glycoproteins pre-extracted with saponin from Chinese hamster ovary (CHO) cells and triton-X100-solubilized. Glycoproteins were affinity-purified by binding to wheat-germ agglutinin. The fractions eluting with N-acetyl D-glucosamine were used.
Alternate Gene Names: CD107b, LGP-B
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3U1
Epitope Location or Sequence:
Uniprot ID: P49130 
Immunogen Sequence: Full length protein
Entrez Gene ID: 100689316 
Additional Characterization:  
Antibody Registry ID: AB_528128 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
UH3 was deposited to the DSHB by Granger, B.L. / Uthayakumar, S. (DSHB Hybridoma Product UH3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular & molecular biology research 41.5 (1995): 405-20.

    IF References
    WB References

    Asparagine-linked oligosaccharides protect Lamp-1 and Lamp-2 from intracellular proteolysis.
    Kornfeld S
    The Journal of biological chemistry 274.43 (1999 Oct 22): 31039-46.

    IP References

    Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
    Granger BL
    Cellular & molecular biology research 41.5 (1995): 405-20.

    All References

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