VM-1

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$40.00
SKU: VM-1-s
View product citations for antibody VM-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: keratinocyte, basal, cell attachment antigen (human)
Hybridoma Cells Available: No
Antigen Species: human
Depositor: Morhenn, V.B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Human
Depositors Notes: Cell binding note: basal cells of human skin and psoriatic lesion skin, basal cell. Immunohistology: carcinoma cells, outer layers of squamous cell carcinoma. Functional effects: inhibits attachment of human keratinocytes to collagen substrate. IHC note: stains frozen sections well.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: keratinocytes from psoriatic plague
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/08A2
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VM-1 was deposited to the DSHB by Morhenn, V.B. (DSHB Hybridoma Product VM-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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  • Initial Publication
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