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View product citations for antibody WE3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: WE3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_531902 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: blastemas sonicates from adult newt
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: wound epithelium & transport/secretory cell protein, 42 kDa
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/15/1990
Antigen Molecular Weight: 42 kDa protein
Depositor: Tassava, R.A.
Antigen Sequence:
Depositor Institution: Ohio State University
Antigen Species: newt
Depositor Notes: Cell binding note: intracellular. Immunohistology: wound epithelium and transport/secretory cells. Specificity note: adult newts (Notophthalmus viridescens). Less reactive to axolotls, not at all to frogs. Immunoblotting: dot blots and Western blots. Functional effects: possible involvement in transport/secretion. IHC note: intracellular antigen appears granular during initial turn-on and then becomes associated with cytoskeleton.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Axolotl, Newt
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
WE3 was deposited to the DSHB by Tassava, R.A. (DSHB Hybridoma Product WE3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Regenerate epithelium and skin glands of the adult newt react to the same monoclonal antibody.
    Gross J
    The Journal of experimental zoology 239.2 (1986 Aug): 229-40.

    All References

    Regenerate epithelium and skin glands of the adult newt react to the same monoclonal antibody.
    Gross J
    The Journal of experimental zoology 239.2 (1986 Aug): 229-40.

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