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View product citations for antibody WE3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: wound epithelium & transport/secretory cell protein, 42 kDa
Hybridoma Cells Available: Yes
Antigen Species: newt
Depositor: Tassava, R.A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Ohio State University
Positive Tested Species Reactivity: Axolotl, Newt
Depositors Notes: Cell binding note: intracellular. Immunohistology: wound epithelium and transport/secretory cells. Specificity note: adult newts (Notophthalmus viridescens). Less reactive to axolotls, not at all to frogs. Immunoblotting: dot blots and Western blots. Functional effects: possible involvement in transport/secretion. IHC note: intracellular antigen appears granular during initial turn-on and then becomes associated with cytoskeleton.
Antigen Molecular Weight: 42 kDa protein
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: blastemas sonicates from adult newt
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
WE3 was deposited to the DSHB by Tassava, R.A. (DSHB Hybridoma Product WE3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Regenerate epithelium and skin glands of the adult newt react to the same monoclonal antibody.
    Gross J
    The Journal of experimental zoology 239.2 (1986 Aug): 229-40.

    All References

    Regenerate epithelium and skin glands of the adult newt react to the same monoclonal antibody.
    Gross J
    The Journal of experimental zoology 239.2 (1986 Aug): 229-40.

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