WE4

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$40.00
SKU: WE4
View product citations for antibody WE4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: wound epithelium & transport / secretory cytoskeletal protein
Hybridoma Cells Available: Yes
Antigen Species: newt
Depositor: Tassava, R.A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Ohio State University
Positive Tested Species Reactivity: Axolotl, Newt
Depositors Notes: Immunohistology note: cytoskeleton. Specificity note: newts & axolotls (weak). Immunoblotting note: yes: non-denaturing. Functional effects: up-regulated in wound epithelium of newts/transport/secretion. IHC note: cytoskeleton.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: blastema of newt
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
WE4 was deposited to the DSHB by Tassava, R.A. (DSHB Hybridoma Product WE4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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