XIIC4

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$40.00
SKU: XIIC4-s
View product citations for antibody XIIC4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: sarcalumenin
Hybridoma Cells Available: No
Antigen Species: rabbit
Depositor: Campbell, K.P.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Canine, Guinea Pig, Mouse, Rabbit
Depositors Notes:
Antigen Molecular Weight: 53 kDa glycoprotein (Sarcalumenin)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: purified rabbit sarcoplasmic reticulum membrane vesicles
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information: RRID:AB_1157899
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
XIIC4 was deposited to the DSHB by Campbell, K.P. (DSHB Hybridoma Product XIIC4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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