Y10b

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$40.00
SKU: Y10b
View product citations for antibody Y10b on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ribosomal RNA (rRNA)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Steitz, J. / Rubel, E.W.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University / University of Washington
Positive Tested Species Reactivity: Chick, Eukaryotes, Human, Mouse, Rat, Squid, Xenopus, Zebrafish
Depositors Notes: Y10b labels large and small subunit of ribosomes in eukaryotic cells. All ribosomal RNAs are reactive - 18S, 5.8S, 23S and maybe 5S. This antibody is an autoantibody that spontaneously developed in an SLE mouse model.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rn18s
Immunogen: ribosomal RNA spontaneous autoantibodies in MRL/lpr mouse strain
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: NA
Entrez Gene ID: 19791 
Additional Characterization:  
Antibody Registry ID: AB_2313703 
Additional Information: RRID:AB_2313703
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Y10b was deposited to the DSHB by Steitz, J. / Rubel, E.W. (DSHB Hybridoma Product Y10b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

23 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication

    Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
    Steitz JA
    Proceedings of the National Academy of Sciences of the United States of America 78.5 (1981 May): 2737-41.

    IF References

    Axonal ribosomes and mRNAs associate with fragile X granules in adult rodent and human brains.
    Fallon JR
    Human molecular genetics 26.1 (2017 Jan 1): 192-209.

    A viral nuclear noncoding RNA binds re-localized poly(A) binding protein and is required for late KSHV gene expression.
    Steitz JA
    PLoS pathogens 7.10 (2011 Oct): e1002300.

    Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
    Seli E
    The Biochemical journal 445.1 (2012 Jul 1): 93-100.

    Tcof1/Treacle is required for neural crest cell formation and proliferation deficiencies that cause craniofacial abnormalities.
    Trainor PA
    Proceedings of the National Academy of Sciences of the United States of America 103.36 (2006 Sep 5): 13403-8.

    A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons.
    Twiss JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.23 (2001 Dec 1): 9291-303.

    Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site.
    Puglisi JD
    Proceedings of the National Academy of Sciences of the United States of America 112.2 (2015 Jan 13): 319-25.

    Increased axonal ribosome numbers is an early event in the pathogenesis of amyotrophic lateral sclerosis.
    van Minnen J
    PloS one 9.1 (2014): e87255.

    Ribosomes in the squid giant axon.
    Martin R
    Neuroscience 107.3 (2001): 527-34.

    Fragile X granules are a family of axonal ribonucleoprotein particles with circuit-dependent protein composition and mRNA cargos.
    Akins MR
    The Journal of comparative neurology 526.1 (2018 Jan 1): 96-108.

    In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site.
    Steitz JA
    Nature structural & molecular biology 13.4 (2006 Apr): 347-53.

    Dendritic localization of the RNA-binding protein HuD in hippocampal neurons: association with polysomes and upregulation during contextual learning.
    Perrone-Bizzozero NI
    Neuroscience letters 371.2-3 (2004 Nov 23): 152-7.

    A viral nuclear noncoding RNA binds re-localized poly(A) binding protein and is required for late KSHV gene expression.
    Steitz JA
    PLoS pathogens 7.10 (2011 Oct): e1002300.

    Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes.
    Boccaccio GL
    Molecular biology of the cell 16.1 (2005 Jan): 405-20.

    Temporal, spatial, and morphologic features of hair cell regeneration in the avian basilar papilla.
    Rubel EW
    The Journal of comparative neurology 417.1 (2000 Jan 31): 1-16.

    Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs.
    Steitz JA
    The EMBO journal 17.12 (1998 Jun 15): 3448-60.

    IHC References
    IP References

    A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons.
    Twiss JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.23 (2001 Dec 1): 9291-303.

    Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
    Steitz JA
    Proceedings of the National Academy of Sciences of the United States of America 78.5 (1981 May): 2737-41.

    A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons.
    Twiss JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.23 (2001 Dec 1): 9291-303.

    OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress.
    Sarnow P
    Molecular and cellular biology 30.8 (2010 Apr): 2006-16.

    In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site.
    Steitz JA
    Nature structural & molecular biology 13.4 (2006 Apr): 347-53.

    Dendritic localization of the RNA-binding protein HuD in hippocampal neurons: association with polysomes and upregulation during contextual learning.
    Perrone-Bizzozero NI
    Neuroscience letters 371.2-3 (2004 Nov 23): 152-7.

    AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2.
    Steitz JA
    Cell 128.6 (2007 Mar 23): 1105-18.

    FFPE References

    A biphasic change in ribosomal conformation during transneuronal degeneration is altered by inhibition of mitochondrial, but not cytoplasmic protein synthesis.
    Rubel EW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 14.4 (1994 Apr): 1994-2008.

    All References

    A biphasic change in ribosomal conformation during transneuronal degeneration is altered by inhibition of mitochondrial, but not cytoplasmic protein synthesis.
    Rubel EW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 14.4 (1994 Apr): 1994-2008.

    Activation of metabotropic glutamate receptors is necessary for transneuronal regulation of ribosomes in chick auditory neurons.
    Hyson RL
    Brain research 809.2 (1998 Nov 2): 214-20.

    Activity-dependent regulation of calcium and ribosomes in the chick cochlear nucleus.
    Hyson RL
    Neuroscience 316. (2016 Mar 1): 201-8.

    Dissecting Torsin/cofactor function at the nuclear envelope: a genetic study.
    Schlieker C
    Molecular biology of the cell 27.25 (2016 Dec 15): 3964-3971.

    A role for inhibition in deafness-induced plasticity of the avian auditory brainstem.
    Hyson RL
    Neuroscience 327. (2016 Jul 7): 10-9.

    Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2.
    Wang Y
    The Journal of comparative neurology 521.5 (2013 Apr 1): 1165-83.

    Axonal ribosomes and mRNAs associate with fragile X granules in adult rodent and human brains.
    Fallon JR
    Human molecular genetics 26.1 (2017 Jan 1): 192-209.

    A viral nuclear noncoding RNA binds re-localized poly(A) binding protein and is required for late KSHV gene expression.
    Steitz JA
    PLoS pathogens 7.10 (2011 Oct): e1002300.

    Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
    Seli E
    The Biochemical journal 445.1 (2012 Jul 1): 93-100.

    Tcof1/Treacle is required for neural crest cell formation and proliferation deficiencies that cause craniofacial abnormalities.
    Trainor PA
    Proceedings of the National Academy of Sciences of the United States of America 103.36 (2006 Sep 5): 13403-8.

    A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons.
    Twiss JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.23 (2001 Dec 1): 9291-303.

    Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site.
    Puglisi JD
    Proceedings of the National Academy of Sciences of the United States of America 112.2 (2015 Jan 13): 319-25.

    Increased axonal ribosome numbers is an early event in the pathogenesis of amyotrophic lateral sclerosis.
    van Minnen J
    PloS one 9.1 (2014): e87255.

    Ribosomes in the squid giant axon.
    Martin R
    Neuroscience 107.3 (2001): 527-34.

    Fragile X granules are a family of axonal ribonucleoprotein particles with circuit-dependent protein composition and mRNA cargos.
    Akins MR
    The Journal of comparative neurology 526.1 (2018 Jan 1): 96-108.

    In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site.
    Steitz JA
    Nature structural & molecular biology 13.4 (2006 Apr): 347-53.

    Dendritic localization of the RNA-binding protein HuD in hippocampal neurons: association with polysomes and upregulation during contextual learning.
    Perrone-Bizzozero NI
    Neuroscience letters 371.2-3 (2004 Nov 23): 152-7.

    Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes.
    Boccaccio GL
    Molecular biology of the cell 16.1 (2005 Jan): 405-20.

    Temporal, spatial, and morphologic features of hair cell regeneration in the avian basilar papilla.
    Rubel EW
    The Journal of comparative neurology 417.1 (2000 Jan 31): 1-16.

    Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs.
    Steitz JA
    The EMBO journal 17.12 (1998 Jun 15): 3448-60.

    Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
    Steitz JA
    Proceedings of the National Academy of Sciences of the United States of America 78.5 (1981 May): 2737-41.

    OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress.
    Sarnow P
    Molecular and cellular biology 30.8 (2010 Apr): 2006-16.

    AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2.
    Steitz JA
    Cell 128.6 (2007 Mar 23): 1105-18.

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