Y10b
Catalog Fields
Antigen: ribosomal RNA (rRNA)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Steitz, J. / Rubel, E.W.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University / University of Washington
Positive Tested Species Reactivity: Chick, Eukaryotes, Human, Mouse, Rat, Squid, Xenopus, Zebrafish
Depositors Notes: Y10b labels large and small subunit of ribosomes in eukaryotic cells. All ribosomal RNAs are reactive - 18S, 5.8S, 23S and maybe 5S. This antibody is an autoantibody that spontaneously developed in an SLE mouse model.
Predicted Species Reactivity:
Gene: Rn18s
Immunogen: ribosomal RNA spontaneous autoantibodies in MRL/lpr mouse strain
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Entrez Gene ID: 19791
Antibody Registry ID: AB_2313703
Additional Information: RRID:AB_2313703
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Y10b was deposited to the DSHB by Steitz, J. / Rubel, E.W. (DSHB Hybridoma Product Y10b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
23 References
Initial Publication
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
Steitz JA
Proceedings of the National Academy of Sciences of the United States of America 78.5 (1981 May): 2737-41.