ZAG 11B9
Catalog Fields
Product Name/ID: ZAG 11B9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618075
Clonality: Monoclonal
Immunogen: zinc alpha-2 glycoprotein (ZAG) [product of the AZGP1 gene]
Clone:
Immunogen Sequence:
Myeloma Strain: P53X68-Ag8
Epitope Mapped: Yes
Antigen Name: zinc alpha-2-glycoprotein (ZAG)
Epitope Location or Sequence: ZAG 11B9 binds to domain 2 of ZAG (aa 183 to end; bp 3-607-891)
Alternate Antigen Name:
Deposit Date: 3/7/2012
Antigen Molecular Weight: 41 kDa
Depositor: Hale, L.P.
Antigen Sequence:
Depositor Institution: Duke University Medical Center
Antigen Species: human
Depositor Notes: Specificity note: reacts with human zinc alpha-2-glycoprotein (not mouse ZAG; other species not tested). ELISA note: ZAG 1B1 & ZAG 11B9 bind to different epitopes and work well together in an antigen capture format. Staining note: works on acetone fixed frozen and formalin fixed paraffin-embedded tissues; works with microwave citrate, if needed.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Zinc alpha-2-glycoprotein (ZAG) is expressed by a variety of epithelial cell types and is secreted in large amount by certain carcinomas, particularly prostate cancer. ZAG has also been implicated in melanin production, cachexia and metabolism. The following papers used two different anti-ZAG antibodies that we had obtained from a collaborator in an antigen capture ELISA. Hybridoma ZAG 1B1 was made subsequently and has never been published.
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ZAG 11B9 was deposited to the DSHB by Hale, L.P. (DSHB Hybridoma Product ZAG 11B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Zinc alpha-2-glycoprotein is expressed by malignant prostatic epithelium and may serve as a potential serum marker for prostate cancer.
Madden JF
Clinical cancer research : an official journal of the American Association for Cancer Research 7.4 (2001 Apr): 846-53.