Catalog Fields
Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit
Available to For-Profits: Yes
Antigen Species: Chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a, kappa light chain
Depositor: Fambrough, D.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian, Canine, Human, Mouse, Mustelid, Ovine
Depositors Institution: Johns Hopkins School of Medicine
Antigen Molecular Weight: 112 kDa
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. Immunoprecipitation: weak. Negative on Porcine.
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Gene: ATP1A1
Alternate Antibody Name: 2F; alpha2F
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Uniprot ID: P09572
Epitope Location or Sequence:
Entrez Gene ID: 396530
Immunogen Sequence: Total protein
Antibody Registry ID: AB_528090
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: Reacts poorly with rat.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a2F was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a2F)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
6 References
Initial Publication
Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump.
Fambrough DM
The Journal of biological chemistry 263.9 (1988 Mar 25): 4347-54.