a2F
Catalog Fields
Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a, kappa light chain
Depositors Institution: Johns Hopkins School of Medicine
Host Species: mouse
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. Immunoprecipitation: weak. Negative on Porcine.
Positive Tested Species Reactivity: Avian, Canine, Human, Mouse, Mustelid, Ovine
Gene: ATP1A1
Antigen Molecular Weight: 112 kDa
Clonality: Monoclonal
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Epitope Mapped: No
Alternate Antibody Name: 2F; alpha2F
Immunogen Sequence: Total protein
Myeloma Strain: SP2/0 Ag8
Additional Information: Reacts poorly with rat.
Uniprot ID: P09572
Entrez Gene ID: 396530
Antibody Registry ID: AB_528090
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a2F was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a2F)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
6 References
Initial Publication
Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump.
Fambrough DM
The Journal of biological chemistry 263.9 (1988 Mar 25): 4347-54.