a2F

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$40.00
SKU: a2F
View product citations for antibody a2F on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian, Canine, Human, Mouse, Mustelid, Ovine
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. Immunoprecipitation: weak. Negative on Porcine.
Antigen Molecular Weight: 112 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ATP1A1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Alternate Gene Names:
Alternate Antibody Name: 2F; alpha2F
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P09572 
Immunogen Sequence: Total protein
Entrez Gene ID: 396530 
Additional Characterization:  
Antibody Registry ID: AB_528090 
Additional Information: Reacts poorly with rat.
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a2F was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a2F)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    WB References
    IHC References
    IP References
    All References

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