Clone ID / Name: a2F
Anti - ATPase, (Na(+) K(+)) alpha-1 subunit
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG2a, kappa light chain
Myeloma Strain: SP2/0 Ag8
Antibody Registry / RRID: AB_528090
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Immunogen Sequence: Total protein
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: Reacts poorly with rat.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Avian, Canine, Human, Mouse, Mustelid, Ovine
Predicted Reactivity: —
Depositor
Depositor Name: D.M. Fambrough
Institution: Johns Hopkins School of Medicine
Deposit Date: October 10, 1990
Depositor Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. Immunoprecipitation: weak. Negative on Porcine.
Recommended Applications
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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