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SKU: a2F
View product citations for antibody a2F on CiteAb

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Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a, kappa light chain
Depositors Institution: Johns Hopkins School of Medicine
Host Species: mouse
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. Immunoprecipitation: weak. Negative on Porcine.
Positive Tested Species Reactivity: Avian, Canine, Human, Mouse, Mustelid, Ovine
Gene: ATP1A1
Antigen Molecular Weight: 112 kDa
Clonality: Monoclonal
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Epitope Mapped: No
Alternate Antibody Name: 2F; alpha2F
Immunogen Sequence: Total protein
Myeloma Strain: SP2/0 Ag8
Additional Information: Reacts poorly with rat.
Uniprot ID: P09572 
Entrez Gene ID: 396530 
Antibody Registry ID: AB_528090 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a2F was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a2F)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    WB References
    IHC References
    IP References
    All References

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