a7C

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$45.00 to $425.00
SKU: a7C
View product citations for antibody a7C on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit (avian)
Available to For-Profits: Yes
Antigen Species: Chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Fambrough, D.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian
Depositors Institution: Johns Hopkins School of Medicine
Antigen Molecular Weight: 112 kDa
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; good for lightly-fixed cells in tissue culture.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Gene: ATP1A1
Alternate Antibody Name: 7C; alpha7C
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Uniprot ID: P09572 
Epitope Location or Sequence:
Entrez Gene ID: 396530 
Immunogen Sequence: Total protein
Antibody Registry ID: AB_528093 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a7C was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a7C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

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