a7C

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$40.00
SKU: a7C
View product citations for antibody a7C on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit (avian)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; good for lightly-fixed cells in tissue culture.
Antigen Molecular Weight: 112 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ATP1A1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Alternate Gene Names:
Alternate Antibody Name: 7C; alpha7C
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P09572 
Immunogen Sequence: Total protein
Entrez Gene ID: 396530 
Additional Characterization:
Antibody Registry ID: AB_528093 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a7C was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a7C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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