a7C

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$40.00
SKU: a7C
View product citations for antibody a7C on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit (avian)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; good for lightly-fixed cells in tissue culture.
Antigen Molecular Weight: 112 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ATP1A1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Alternate Gene Names:
Alternate Antibody Name: 7C; alpha7C
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P09572 
Immunogen Sequence: Total protein
Entrez Gene ID: 396530 
Additional Characterization:  
Antibody Registry ID: AB_528093 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a7C was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a7C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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