anti-CD38 (HB-7)
Catalog Fields
Clone ID/Product Name: anti-CD38 (HB-7)
Available to For-Profits: No
Alternate Antibody Name: H7
Gene Symbol: CD38
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_3105832
Uniprot ID: P28907
Entrez Gene ID: 952
Clonality: Monoclonal
Immunogen: Human B cell line BJAB
Clone:
Immunogen Sequence: Full-length sequence
Myeloma Strain: P3X63-Ag8.653
Epitope Mapped: No
Antigen Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38)
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 6/27/2024
Antigen Molecular Weight: 45 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Human
Depositor Notes: CD38 exists as both a soluble and a cell surface molecule, and possesses enzymatic activity in both normal and pathologic states. CD31 (PECAM-1) is the natural ligand for CD38. CD38 is a naïve B cell marker and is re-expressed late in plasma cell differentiation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: Surface plasmon resonance KD equals 3.01E?12 PMID: 36239134.
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-CD38 (HB-7) was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product anti-CD38 (HB-7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
2 References
Initial Publication
Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation.
Cooper MD
Tissue antigens 24.3 (1984 Sep): 140-9.