anti-GLD-1

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$50.00
SKU: anti-GLD-1-p

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Available: 72

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: anti-GLD-1
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: gld-1
Ab Isotype: Rabbit IgG
Gene Name:
Antibody Registry ID: AB_3105831 
Uniprot ID: Q17339 
RRID:  
Entrez Gene ID: 172532 
Clonality: Polyclonal
Immunogen: Recombinant GST-GLD-1
Clone:
Immunogen Sequence: Full sequence
Myeloma Strain: N.A.
Epitope Mapped: No
Antigen Name: Female germline-specific tumor suppressor gld-1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/26/2024
Antigen Molecular Weight: 50.5 kDa
Depositor: Kimble, J.E.
Antigen Sequence:
Depositor Institution: University of Wisconsin, Madison
Antigen Species: C. elegans
Depositor Notes: RNA-binding protein represses translation specifically to germ line-oocytes and is essential for oogenesis.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: C. elegans
Additional Information: The specificity of this antibody has been validated using gld-1 KO strains. PMID: 878068. Recommended 4% paraformaldehyde fixation and 1:200 serum dilution. PMID: 38290520
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-GLD-1 was deposited to the DSHB by Kimble, J.E. (DSHB Hybridoma Product anti-GLD-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    WB References

    Visual evoked responses, EEG's and reaction time during a normoxic saturation dive, NISAT I.
    Luria SM
    Undersea biomedical research 4.2 (1977 Jun): 131-45.

    Changing views of NMR: automated high-resolution NMR with a sample changer.
    McEnroe FJ
    Analytical chemistry 61.2 (1989 Jan 15): 107A-111A.

    IP References
    Gel Supershift References
    All References

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